Saturday, July 13, 2013

Understanding ELISA Kits


Enzyme-linked immunosorbent assay (ELISA) is a biochemical procedure in immunology that is primarily used for determining the presence of an antigen or an antibody in a sample. For years, ELISA has been utilized as a diagnostic tool in plant pathology and medicine. It has test kits that are used for cancer, autoimmune diseases, fertility, thyroid, steroid, infectious diseases and parasitology, allergy, and diabetes assays.

One of the ELISA test kits for cancer is Prostatic Acid Phosphatase (PAP), which is used for measuring the prostatic acid phosphatase (PAP) in the serum and plasma of a human. The components of the kit are designed for use as an integral unit only. Therefore, the components from various lots must be separated. The Diagnostic Automation PSA Enzyme Immunoassay is also a test kit for cancer. It is especially designed for quantitative detection of prostate-specific antigen (PSA) in the human serum.

Another example of test kit is the Human Allergen Specific IgE ELISA Assay, which is used to quantitate and qualitate the allergen specific human Immunoglobulin E. The total IgE of the specimen is determined before performing allergen specific IgE assays. The Human Growth Hormone (HGH), another type of test kit is used for testing the fertility of a woman. It determines the human growth hormone concentration within the human serum.

There are also test kits for infectious diseases and one of these is the Bordetella pertussis IgA, which is intended to be used for determining the specific IgA antibodies against the Bordetella pertussis in the plasma and serum. The assay is designed for in-vitro diagnostic test only. Therefore, further tests need to be taken into account.

There are several manufacturers of ELISA kits and one of these is Shanghai PZ Bio-tech Co., Ltd. in China. Another ELISA Kit Manufacturer is Taiwan Advance Bio-Pharm Inc., which is based in Taiwan. Other manufacturers of ELISA kits is the Wuhan Boster Biological Technology, Ltd. (China), Green House Pharm Pte Ltd (Singapore), and Biocare Diagnostics Limited (China).

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